From the Pico series-Pico, Nene, Darnell, Piconjo, Uberkid, Bear, Pico's Cousin.

From the Alien Hominid series-Alien Hominid, FBI Agent, The Kid, Robot #1(first boss), Robot #2 (second boss)

From the rest of the Tom Fulp creation series-Mason(mason's bubble blast), Chainsaw Guy(chainsaw the children), Samurai A., P-Bot, A-Bot, Assassin(from the assassin collection of course), Tankman, Eddie(from megaman), Salad Fingers

From the crazy mind of MindChamber and Luis-Hank, Alloy, Convict

From the creation of AlanMidas-Eskimo Bob, Alfonzo

From the creation of Egoraptor-Snake, Master Chief, Samus(Awesome Series)

From section 8 studios-Hooch Pandersnatch(also known as the Pimp from "Pimp's quest"), Rudy Youngblood(Pimp's quest)

Bonus Characters added-Megaman, Roll, Pac-Man, Batman, Assassin(female version), Ninja Joe(Shinobi), Nina Jill(own creation), Stick Man, Dark Shin Akuma
Well there you go, 40 characters confirmed for my big flash project, I can't guarantee an accurate date on when it will be completed, I don't think I can even estimate it. Wish me luck and I hope I create a fantastic game you all can enjoy! Later.